Volume 9, Issue 1, March 2020, Page: 10-13
Antimicrobial Activity of Endophytic Fungi Isolated from hysalis angulata L. Plant
Utami Sri Hastuti, Microbiology Laboratory, Department of Biology, State University of Malang, Malang, Indonesia
Dwi Rahmawati, Microbiology Laboratory, Department of Biology, State University of Malang, Malang, Indonesia
Ria Yustika Sari, Microbiology Laboratory, Department of Biology, State University of Malang, Malang, Indonesia
Sugi Hartono, Microbiology Laboratory, Department of Biology, State University of Malang, Malang, Indonesia
Chomisatut Thoyibah, Microbiology Laboratory, Department of Biology, State University of Malang, Malang, Indonesia
Fitria Maulita, Microbiology Laboratory, Department of Biology, State University of Malang, Malang, Indonesia
Faiza Nur Imawati Ningsih, Microbiology Laboratory, Department of Biology, State University of Malang, Malang, Indonesia
Received: Jan. 6, 2020;       Accepted: Feb. 6, 2020;       Published: Feb. 14, 2020
DOI: 10.11648/j.jenr.20200901.12      View  214      Downloads  68
Abstract
Some endophytic fungi live in plant tissue and does not caused any damage on their host plant. The endophytic fungi could produce antimicrobial secondary metabolites. Some endophytic fungi have been isolated from a medicinal plant Physalis angulata, i.e: Penicillium verrucosum, Colletotrichum alienum, Fusarium subglutinans, Aspergillus nidulans, Mycelia sterilia 1, Mycelia sterilia 2, and Rhizoctonia sp. The purpose of this research are: 1) to examine the antimicrobial activity of each endophytic fungi species toward Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and, 2) to determine the endophytic fungi species that have highest antimicrobial activity. Each endophytic fungi isolates were inoculated in Potato Dextrose Broth (PDB) medium and shaked in 120 rpm, during 7 x 24 hours, then the broth culture centrifugated in the rate of 3000 rpm for 10 minutes. The supernatants from each endophytic fungi were treat to the bacteria to know the antimicrobial activity by agar diffusion method. Afterwards the antibaterial effect of each endophytic fungi species were measured. Then the highest antimicrobial effect were determined. The research results shows that: 1) each endophytic fungi species have antimicrobial activity towards B. subtilis, E. coli, S. aureus, and; 2) Penicillium verucossum secondary metabolites have the highest antimicrobial activity.
Keywords
Endophytic Fungi, Secondary Metabolites, Antimicrobial, P. Angulata
To cite this article
Utami Sri Hastuti, Dwi Rahmawati, Ria Yustika Sari, Sugi Hartono, Chomisatut Thoyibah, Fitria Maulita, Faiza Nur Imawati Ningsih, Antimicrobial Activity of Endophytic Fungi Isolated from hysalis angulata L. Plant, Journal of Energy and Natural Resources. Vol. 9, No. 1, 2020, pp. 10-13. doi: 10.11648/j.jenr.20200901.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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